EPA-RFP

 

Susceptibility of Antibacterial Chemicals on Pseudomonas aeruginosa  

 

Table of Contents:

1) Abstract

2) Research Plan

3) Resume

4) Current and Pending Support

5) Budget

6) Budget Justification

7) Quality Assurance Statement

 

Brady, Clayton Neal

915 Laurel Ave.

Cookeville, TN 38501

931-372-5992

cnbrady21@tntech.edu

U.S. Citizen

Hometown: Columbia, TN

 

Bachelor’s Degree in Health Science Biology

May 2005

 

Undergraduate

Tennessee Technological University

Cookeville, TN 38505

Department of Biology

August 2002-December 2002

 

Research Plan

 

Project Cost: Free! Materials graciously provided by the Biology Dept. of Columbia State Community College.

 

Project Summary

 The objective of my research is to determine if chemicals commonly found in the antibacterial sprays we use so abundantly today can in fact render potentially hazardous bacteria, such as Pseudomonas aeruginosa, non-viable.

 

 

 

Methods and Materials

This experiment required Petri dishes, a synthetic stock of Pseudomonas aeruginosa, an inoculation loop, and a sterilizer (incinerator) for streaking the plates.  The chemicals I used were Isopropyl Alcohol, 1 M Hydrochloric Acid, 5 % Hypochlorite Bleach, and Vesphene. All of these chemicals are commonly used to disinfect areas contaminated with bacteria.  In order to have the bacteria to grow I needed an incubation device.  A ruler was needed to measure the zone of inhibition around the discs. Finally, an autoclave was required to sterilize the tools after use.

The method used in my experiment was the Mueller Hinton / Pseudomonas Agar Disc Diffusion Assay Tests. (Goss, 62-65)  Using aseptic techniques, the experiment began with taking an inoculating loop, sterilizing it by way of heating it, and obtaining a sample of the bacteria being used.  The bacteria were then exposed to discs dipped in each of the four chemicals.  The dishes were separated into quadrants for each of the four chemicals tested.  The bacteria were given a full day to grow and on the third day the zone of inhibition was observed and measured for results.

 

Expected Results and Benefits

      It is expected that, after careful aseptic experimentation, Pseudomonas aeruginosa will be rendered non-viable by one or more of the chemicals.

      Benefits of this experiment will include a better understanding of how to properly disinfect this bacteria as well as a better understanding of the bacteria itself.

Keywords

Pseudomonas aeruginosa

Biofilm

Antimicrobial

Antibacterial

 

     Resume

Clayton Neal Brady

 

QUALIFICATIONS

I am an outgoing and hard working young man who believes in honesty and fair play.  I am dependable, trustworthy and strive to show excellence in all aspects of my life.  When I start a task I am not satisfied until it is completed in a professional and timely manner. I have roughly two years experience providing service to customers and members and feel that it is a strong part of my character.

 

EDUCATION

2003-Present  Currently enrolled-Tennessee Technological University

2001-2003       Completed transfer program - Columbia State Community College

1997-2001       Honors Diploma, Santa Fe High School

 

 

Educational

 Institution

 

Grade Point

Average

 

Columbia State Community College

 

3.6

 

Santa Fe High School

 

                          3.7

 

EMPLOYMENT

Nov. 2002-June 2003                            Non-Certified Optician, Wal-Mart Vision Center

Fitted customers for glasses and contacts.  Informed customers about proper eyeglass and contact care.  Sold lenses and frames according to customers’ needs and lifestyles.  Provided customer service that included repairing glasses and listening to comments and complaints about the vision center and trying to address all concerns in a professional manner.

 

Feb. 2002-Nov. 2002                           Site Director, Maury County YMCA

Kept an accurate record of all payments.  In charge of all nutritional and developmental needs of children while at the after school program.  Addressed concerns of parents in regards to safety of children as well as

    general member concerns and comments about the YMCA.

 

Feb. 2001-Feb.2002                            Site Assistant, Maury County YMCA

                              Assisted Site Director in all aspects of after school childcare.

      

The requirements of the degree that I am currently seeking are outlined in the Tennessee Technological University Undergraduate Catalogue. A degree in Health Science Biology demands an emphasis in biology and chemistry and a total of 120 course hours in order to graduate.

Current and Pending Support

Dr. Karen Kendell-Fite

Biology Dept. Of COSCC

Budget

All supplies were graciously provided by Dr. Karen Kendell-Fite of the Biology Department of Columbia State Community College.

 

 

Budget Justification

N/A

 

Quality Assurance Statement

            Being a health science biologist, I must make strides to help keep and healthy, aseptic environment. The world is full of harmful, resistant bacteria that must be ridded of at all times. This degree will allow me to perform laboratory procedures in most hospitals, such as cultures, tube dilutions, etc., and will also give me a great deal of background material before applying to medical school, which will be essential in keeping a clean practice.

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