Susceptibility of Antibacterial Chemicals on
Pseudomonas aeruginosa

1^st Progress Report

Research performed by:

Clayton Brady

Health Science Biology Major

Tennessee Technological University

Clayton Brady

915 Laurel Ave Apt. Q1E

Cookeville TN 38501

April 21, 2004

Dr. Morgan
Tennessee Technological University

Dear Mr. Smoker:

I am pleased to have the opportunity to discuss the status of my experiment regarding the susceptibility of antibacterial chemicals on Pseudomonas aeruginosa. The need for reliable chemicals to disinfect surfaces contaminated with bacteria is growing each day. My research should help solve the problem of how to kill resistant bacteria.

The attached progress report my status on the project. Included in the report is a table of contents, a list of latest tables and figures and a project summary.

Sincerely,



Clayton N. Brady, Researcher
Tennessee Tech University

Attached: Progress Report

Table of contents

1. Project Summary

2. Progression status

3. Appendices

Project Summary

Antimicrobial agents are used in our homes as well as hospitals to kill or hinder dangerous bacteria.  Bacteria can be resistant to many of these agents.  Pseudomonas aeruginosa will be used as a test subject for this experiment for the because of its resistance to many different agents.  This subject is difficult to isolate and will prove to be a more than formidable foe for these antibacterial agents.  The objective of my research is to determine if the antibacterial sprays we use so abundantly today can in fact render potentially hazardous bacteria, such as Pseudomonas aeruginosa, non-viable.

Progression Status

Thus far I have obtained my synthetic stock of Pseudomonas aeruginosa and plan to inoculate it next Monday. I will then separate the agar into quadrants and place the dipped discs on the agar. The bacteria will be given all day Tuesday to grow and I will observe and record my results on Wednesday.

As you know I started the project wanting to test cleaners against the bacteria, but upon closer look I observed that most of the cleaners had the same ingredients so I decided to use common antibacterial chemicals to test against the bacteria.

So far everything is going to plan and looks good. Results should be calculated by the end of next week.

I recommend doing a similar project at a later date testing Pseudomonas that has developed biofilms.

Appendices

1. The chart that will be used to record data.

Pseudomonas aeruginosa Disk Diffusion Chart
Disc Diffusion Assays
	Susceptible	Resistant	Zone of Inhibition
Isopropyl Alcohol
1 M HCl
5 % hypochlorite bleach
Vesphene

2. Schedule of Remaining Tasks

Task	Estimated date of completion	Estimated time for completion
Pour agar	4-15-04	30 minutes
Inoculate bacteria	4-15-04	30 minutes
Perform disc diffusion tests	4-15-04	24-36 hours
Measure Zones of Inhibition	4-17-04	30 minutes

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